Composite

Part:BBa_K371018

Designed by: Yang Zhang   Group: iGEM10_USTC   (2010-10-11)

MPF+RFP+MSF

This composite part is RFP protein used for protein fusion according to BBF [RFC53][http://bbf.openwetware.org/RFC.html#BBF_RFC_53:_USTC_MetaPart_Assembly_Standard_--_Extending_RFC_10_to_Enable_Scarless_Protein_Fusion_with_Type_IIS_Restriction_Enzyme_EarI_and_SapI]. It origin from BioBrick BBa_E0043 with MPF and MSF at the two end of its sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 565
    Illegal AgeI site found at 677
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3


[edit]
Categories
Parameters
n/aMPF+RFP+MSF